지방흡입 Things To Know Before You Buy
지방흡입 Things To Know Before You Buy
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Do you have a protocol or advised antibodies to carry out ICC staining on my completely differentiated ALI cultures?
Mix gently before transferring cells to your conical tube made up of ten mL of pre-warmed wash medium. Be aware: Clean the pipette suggestion and cryovial with the wash medium no less than three instances to help make improvements to recovery.
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Utilizing a P1000 pipette, Carefully triturate BLOs within the apical chamber somewhere around 3 periods whilst circling the suggestion around the insert. Transfer contents into a fifteen mL conical tube.
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Alternatively, dissociate organoids utilizing Light Cell Dissociation Reagent (GCDR) without the need of prior retrieval of organoids within the Matrigel® dome. This can be unquestionably faster but bears the chance of inadequate cell dissociation because of issues with Matrigel® and could be additional stressful towards the cells.
Assess the standing by checking a droplet and stopping the dissociation when close to 80% on the cell suspension is single-celled. Steer clear of above-digestion.
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Take 자가지방이식 note: PFA fixation can be thought of for examining intracellular markers or accomplishing Investigation in a later on time.
Clear away supernatant and resuspend cells in FACS buffer. Notice: It's important to quench the dissociation reagent by utilizing the identical or double the volume with the dissociation reagent.